The Human DKK2 (Dickkopf-related protein 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of DKK2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.DKK2 is a critical protein involved in the regulation of Wnt signaling pathway, influencing cell differentiation and development. Dysregulation of DKK2 has been implicated in various diseases including cancer, osteoporosis, and Alzheimer's disease, highlighting its importance as a potential biomarker for these conditions.
With its high sensitivity and specificity, the Human DKK2 CLIA Kit is an essential tool for scientists and researchers studying the role of DKK2 in disease pathogenesis and developing targeted therapies. Order now to advance your research and gain valuable insights into the molecular mechanisms underlying these complex diseases.
Product Name:
Human DKK2 (Dickkopf Related Protein 2) CLIA Kit
SKU:
HUES00521
Target:
Human DKK2 (Dickkopf Related Protein 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DKK2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DKK2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DKK2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DKK2. You can calculate the concentration of Human DKK2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-116
98-115
86-98
Average (%)
110
106
91
1:4
Range (%)
98-110
101-116
101-119
Average (%)
104
108
108
1:8
Range (%)
99-113
85-100
87-98
Average (%)
105
91
93
1:16
Range (%)
96-113
87-100
102-116
Average (%)
104
93
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.35
305.37
807.52
99.78
325.47
736.97
Standard deviation
12.45
33.71
57.09
11.85
37.72
50.34
C V (%)
12.66
11.04
7.07
11.88
11.59
6.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DKK2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DKK2 in samples. No significant cross-reactivity or interference between Human DKK2 and analogues was observed.
