The Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit is a powerful tool for the quantitative measurement of DNMT3A levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reliable results, making it an invaluable asset for a variety of research studies.DNMT3A is a key enzyme involved in the methylation of DNA, playing a vital role in regulating gene expression and maintaining genomic stability. Dysregulation of DNMT3A has been linked to various diseases, including cancer and developmental disorders, making it a valuable target for research and potential therapeutic interventions.
By utilizing the Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit, researchers can gain valuable insights into the role of DNMT3A in disease pathogenesis and progression, opening up new avenues for understanding and treating these complex conditions. Elevate your research with this cutting-edge ELISA kit from Assay Genie.
Product Name:
Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit
Product Code:
HUDL00856
Size:
96 Assays
Target:
DNMT3A
Synonyms:
DNMT3A2, M.HsaIIIA, DNA methyltransferase HsaIIIA
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
