Description
Human DNA2 Recombinant Protein (N-His)
Human DNA2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Arg599, with an N-terminal His tag. Predicted molecular weight: 70.85 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA replication ATP-dependent helicase/nuclease DNA2 is a ~120 kDa protein. Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. For research use only.
Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities.
| Product Name: | Human DNA2 Recombinant Protein (N-His) |
| SKU: | GXRP04887 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DNA replication ATP-dependent helicase-like homolog, KIAA0083, hDNA2, DNA2, DNA2L, DNA replication ATP-dependent helicase/nuclease DNA2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Arg599 |
| Calculated MW: | 70.85 kDa |
| UniProt: | P51530 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
