Description
Human DNAJB14 Recombinant Protein (N-His-SUMO)
Human DNAJB14 Recombinant Protein (N-His-SUMO) is a recombinant protein expressed in E. coli, spanning Ser81-Thr171, with an N-terminal His-SUMO tag. Predicted molecular weight: 23.53 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DnaJ homolog subfamily B member 14 (DNAJB14) is a ~42 kDa protein. Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. For research use only.
Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB12, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB12 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.
| Product Name: | Human DNAJB14 Recombinant Protein (N-His-SUMO) |
| SKU: | GXRP04468 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DNAJB14, DnaJ homolog subfamily B member 14 |
| Tag: | N-His-SUMO |
| Expression Host: | E. coli |
| Protein Length: | Ser81-Thr171 |
| Calculated MW: | 23.53 kDa |
| UniProt: | Q8TBM8 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
