Description
Human DNMT3B Recombinant Protein (N-His)
Human DNMT3B Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Ser100, with an N-terminal His tag. Predicted molecular weight: 13 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA (cytosine-5)-methyltransferase 3B (DNMT3B) is a ~95 kDa protein. Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. For research use only.
DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. 1. van den Boogaard, ML. et al. (2016) American journal of human genetics 98, 1020-1029. PMID: 27153398
| Product Name: | Human DNMT3B Recombinant Protein (N-His) |
| SKU: | GXRP02993 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | M.HsaIIIB, DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, DNMT3B |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Ser100 |
| Calculated MW: | 13 kDa |
| UniProt: | Q9UBC3 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
