Human Dynein, Axonemal, Heavy Chain 11 (DNAH11) ELISA Kit
The Human Dynein Axonemal Heavy Chain 11 (DNAH11) ELISA Kit is a reliable and accurate tool for detecting levels of DNAH11 in human samples. This kit is specifically designed for use with serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for precise and reproducible results.DNAH11 is a critical component of dynein, a motor protein involved in cell motility and ciliary movement. Dysregulation of DNAH11 has been linked to various diseases including primary ciliary dyskinesia, male infertility, and respiratory diseases.
By measuring DNAH11 levels, researchers can gain valuable insights into these conditions and potential therapeutic targets.With its ease of use and robust performance, the Human DNAH11 ELISA Kit is an essential tool for studying the role of DNAH11 in health and disease, making it a valuable asset for researchers in the field of molecular biology and medicine.
Product Name:
Human Dynein, Axonemal, Heavy Chain 11 (DNAH11) ELISA Kit
Product Code:
HUDL00847
Size:
96 Assays
Target:
DNAH11
Synonyms:
DNAHBL, DNAHC11, DNHBL, DPL11, Dynein,Heavy Chain Beta-Like, Axonemal beta dynein heavy chain 11, Ciliary dynein heavy chain 11
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
