Description
Human EARS2 Recombinant Protein (N-His)
Human EARS2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly247-Gln496, with an N-terminal His tag. Predicted molecular weight: 53.59 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Nondiscriminating glutamyl-tRNA synthetase EARS2, mitochondrial is a ~58 kDa protein. Non-discriminating glutamyl-tRNA synthetase that catalyzes aminoacylation of both mitochondrial tRNA(Glu) and tRNA(Gln) and participates in RNA aminoacylation for mitochondrial protein translation. For research use only.
Attachs glutamate to tRNA(Glu) or tRNA(Gln) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) or tRNA(Gln). In vitro, cytoplasmic tRNA(Gln) is slightly glutamylated, but with low activity.
| Product Name: | Human EARS2 Recombinant Protein (N-His) |
| SKU: | GXRP03229 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | KIAA1970, Glutamyl-tRNA synthetase, EARS2, Probable glutamate--tRNA ligase, mitochondrial, GluRS |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly247-Gln496 |
| Calculated MW: | 53.59 kDa |
| UniProt: | Q5JPH6 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |