Description
Human EEF1A1 Recombinant Protein (N-His)
Human EEF1A1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Pro241, with an N-terminal His tag. Predicted molecular weight: 28.85 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Elongation factor 1-alpha 1 (EEF1A1) is a ~50 kDa protein. Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. For research use only.
Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. Also plays a role in cytoskeleton organization by promoting actin bundling.
| Product Name: | Human EEF1A1 Recombinant Protein (N-His) |
| SKU: | GXRP01928 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EEF1A, Elongation factor Tu, Leukocyte receptor cluster member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Eukaryotic elongation factor 1 A-1, EF-Tu, EF1A, eEF1A-1, LENG7, EEF1A1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Pro241 |
| Calculated MW: | 28.85 kDa |
| UniProt: | P68104 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
