Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit
The Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit is meticulously crafted to enable the precise and quantitative detection of EGFR2 levels in diverse human biological samples. Epidermal Growth Factor Receptor 2 is a critical biomarker known for its role in cell proliferation, differentiation, and various cellular processes, particularly in cancer development and progression. Accurate quantification of EGFR2 is crucial for understanding its involvement in tumorigenesis, prognostic evaluation, and the development of targeted therapeutic strategies. Our ELISA kit offers exceptional sensitivity and specificity, ensuring robust and reproducible results in measuring EGFR2 concentrations. With stringent quality control measures in place, this kit guarantees reliable performance and ease of use, catering to both research investigations and clinical applications. Rely on Assay Genie's Human EGFR2 ELISA Kit for the accurate and dependable quantification of this pivotal biomarker in your scientific studies.
Product Name:
Human EGFR2 (Epidermal Growth Factor Receptor 2) ELISA Kit
SKU:
AEES00119
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
