Description
Human EGLN1 Recombinant Protein (N-His)
Human EGLN1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala190-Phe426, with an N-terminal His tag. Predicted molecular weight: 28.85 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Egl nine homolog 1 (EGLN1) is a ~46 kDa protein. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. For research use only.
Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
| Product Name: | Human EGLN1 Recombinant Protein (N-His) |
| SKU: | GXRP04604 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | C1orf12, SM-20, PHD2, Egl nine homolog 1, HPH-2, EGLN1, HIF-prolyl hydroxylase 2, HIF-PH2, Prolyl hydroxylase domain-containing protein 2, Hypoxia-inducible factor prolyl hydroxylase 2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala190-Phe426 |
| Calculated MW: | 28.85 kDa |
| UniProt: | Q9GZT9 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
