Description
Human EIF1 Recombinant Protein (C-His)
Human EIF1 Recombinant Protein (C-His) is a recombinant protein expressed in E. coli, spanning Met1-Phe113, with a C-terminal His tag. Predicted molecular weight: 13.80 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Eukaryotic translation initiation factor 1 is a ~12 kDa protein. Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. For research use only.
Together with eIF1A (EIF1AX), EIF1 facilitates scanning and is essential for start codon recognition on the basis of AUG nucleotide context and location relative to the 5'-cap. Participates to initiation codon selection by influencing the conformation of the 40S ribosomal subunit and the positions of bound mRNA and initiator tRNA; this is possible after its binding to the interface surface of the platform of the 40S ribosomal subunit close to the P-site. Together with eIF1A (EIF1AX), also regulates the opening and closing of the mRNA binding channel, which ensures mRNA recruitment, scanning and the fidelity of initiation codon selection. Continuously monitors and protects against premature and partial base-pairing of codons in the 5'-UTR with the anticodon of initiator tRNA.
| Product Name: | Human EIF1 Recombinant Protein (C-His) |
| SKU: | GXRP11300 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | A121, EIF1, Eukaryotic translation initiation factor 1, Protein translation factor SUI1 homolog, SUI1, Sui1iso1, eIF1 |
| Tag: | C-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Phe113 |
| Calculated MW: | 13.80 kDa |
| UniProt: | P41567 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
