Description
Human EIF1AX Recombinant Protein (N-His)
Human EIF1AX Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn19-Ile144, with an N-terminal His tag. Predicted molecular weight: 16.72 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Eukaryotic translation initiation factor 1A, X-chromosomal is a ~16 kDa protein. Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. For research use only.
This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B.
| Product Name: | Human EIF1AX Recombinant Protein (N-His) |
| SKU: | GXRP09744 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Eukaryotic translation initiation factor 1A, X-chromosomal; eIF-1A X isoform; eIF1A X isoform; Eukaryotic translation initiation factor 4C; eIF-4C; EIF1AX; EIF1A; EIF4C |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn19-Ile144 |
| Calculated MW: | 16.72 kDa |
| UniProt: | P47813 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
