Description
Human EIF2AK2 Recombinant Protein (N-His)
Human EIF2AK2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser224-Ile502, with an N-terminal His tag. Predicted molecular weight: 34.35 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Interferon-induced, double-stranded RNA-activated protein kinase (EIF2AK2) is a ~62 kDa protein. IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection. For research use only.
Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2-alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteasomal degradation.
| Product Name: | Human EIF2AK2 Recombinant Protein (N-His) |
| SKU: | GXRP03399 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Protein kinase R, PRKR, Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, Protein kinase RNA-activated, EIF2AK2, Tyrosine-protein kinase EIF2AK2, p68 kinase, PKR, P1/eIF-2A protein kinase, Interferon-inducible RNA-dependent protein kinase, eIF-2A protein kinase 2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser224-Ile502 |
| Calculated MW: | 34.35 kDa |
| UniProt: | P19525 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
