Description
Human EIF2D Recombinant Protein (N-His)
Human EIF2D Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys45-Lys182, with an N-terminal His tag. Predicted molecular weight: 17.25 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Eukaryotic translation initiation factor 2D (EIF2D) is a ~64 kDa protein. Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. For research use only.
The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits.
| Product Name: | Human EIF2D Recombinant Protein (N-His) |
| SKU: | GXRP04370 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | LGTN, Ligatin, HCA56, Hepatocellular carcinoma-associated antigen 56, Eukaryotic translation initiation factor 2D, eIF2d, EIF2D |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys45-Lys182 |
| Calculated MW: | 17.25 kDa |
| UniProt: | P41214 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
