Description
Human EIF4A3/DDX48 Recombinant Protein (C-His)
Human EIF4A3/DDX48 Recombinant Protein (C-His) is a recombinant protein expressed in E. coli, spanning Phe40-Ile411, with a C-terminal His tag. Predicted molecular weight: 44.89 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Eukaryotic initiation factor 4A-III (EIF4A3/DDX48) is a ~46 kDa protein. ATP-dependent RNA helicase. For research use only.
Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD).
| Product Name: | Human EIF4A3/DDX48 Recombinant Protein (C-His) |
| SKU: | GXRP03690 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ATP-dependent RNA helicase eIF4A-3, eIF-4A-III, Eukaryotic translation initiation factor 4A isoform 3, Eukaryotic initiation factor 4A-like NUK-34, Nuclear matrix protein 265, hNMP 265, KIAA0111, DDX48, NMP 265, eIF4A-III, Eukaryotic initiation factor 4A-III, ATP-dependent RNA helicase DDX48, EIF4A3, DEAD box protein 48 |
| Tag: | C-His |
| Expression Host: | E. coli |
| Protein Length: | Phe40-Ile411 |
| Calculated MW: | 44.89 kDa |
| UniProt: | P38919 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
