The Human ELA1 (Elastase-1) Pancreatic ELISA Kit is specially designed to accurately measure the levels of ELA1 in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it the perfect choice for a variety of research applications.ELA1, also known as pancreatic elastase-1, is an enzyme that plays a critical role in the breakdown of proteins in the digestive system. Abnormal levels of ELA1 have been associated with conditions such as pancreatitis, cystic fibrosis, and inflammatory bowel disease, making it a valuable biomarker for studying these disorders and developing potential treatments.
This ELISA kit is easy to use and includes all the necessary components for performing the assay. With its accurate and precise measurements, researchers can confidently study the role of ELA1 in various diseases and gain a better understanding of its impact on human health.
Product Name:
Human ELA1 (Elastase 1, Pancreatic) ELISA Kit
SKU:
HUES03572
Target:
Human ELA1 (Elastase 1, Pancreatic )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ELA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ELA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ELA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ELA1. You can calculate the concentration of Human ELA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
99-114
88-99
Average (%)
93
105
93
1:4
Range (%)
92-106
80-93
90-101
Average (%)
98
85
95
1:8
Range (%)
87-100
86-100
86-101
Average (%)
94
92
93
1:16
Range (%)
90-105
81-94
81-93
Average (%)
96
87
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.25
3.59
0.57
1.35
3.49
Standard deviation
0.03
0.07
0.13
0.04
0.06
0.11
C V (%)
5.56
5.6
3.62
7.02
4.44
3.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ELA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ELA1 in samples. No significant cross-reactivity or interference between Human ELA1 and analogues was observed.
