The Human ELA1 (Elastase-1) Pancreatic ELISA Kit is specially designed to accurately measure the levels of ELA1 in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it the perfect choice for a variety of research applications.ELA1, also known as pancreatic elastase-1, is an enzyme that plays a critical role in the breakdown of proteins in the digestive system. Abnormal levels of ELA1 have been associated with conditions such as pancreatitis, cystic fibrosis, and inflammatory bowel disease, making it a valuable biomarker for studying these disorders and developing potential treatments. This ELISA kit is easy to use and includes all the necessary components for performing the assay. With its accurate and precise measurements, researchers can confidently study the role of ELA1 in various diseases and gain a better understanding of its impact on human health.
Product Name:
Human ELA1 (Elastase 1, Pancreatic) ELISA Kit
SKU:
HUES03572
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
