The Human ELN (Elastin) CLIA Kit is specifically designed for the accurate detection of elastin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research purposes.Elastin is a vital protein that is involved in maintaining the elasticity and structure of various tissues in the body, including blood vessels, skin, and lungs. Changes in elastin levels have been linked to diseases such as cardiovascular disorders, pulmonary conditions, and aging-related issues, making it a significant biomarker for studying these health concerns and exploring potential treatment options.
With its advanced technology and reliable performance, the Human ELN (Elastin) CLIA Kit is an essential tool for researchers and healthcare professionals looking to investigate the role of elastin in various physiological and pathological processes. Order your kit now to accelerate your research and uncover new insights into the importance of elastin in human health.
Product Name:
Human ELN (Elastin) CLIA Kit
SKU:
HUES00673
Target:
Human ELN (Elastin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
56.25 ng/mL
Detection range:
93.75-6000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ELN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ELN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ELN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ELN. You can calculate the concentration of Human ELN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
87-103
86-98
Average (%)
99
94
92
1:4
Range (%)
91-105
88-104
100-118
Average (%)
98
95
108
1:8
Range (%)
88-103
91-105
88-100
Average (%)
95
99
93
1:16
Range (%)
92-107
91-107
96-107
Average (%)
100
99
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
97-112
102
Cell culture media (n=5)
102-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
321.0
543.85
2645.01
339.13
561.97
2411.78
Standard deviation
34.44
43.29
204.46
36.56
47.99
252.03
C V (%)
10.73
7.96
7.73
10.78
8.54
10.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ELN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ELN in samples. No significant cross-reactivity or interference between Human ELN and analogues was observed.
