Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit
The Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit is expertly crafted for the precise and quantitative assessment of EMMPRIN/CD147 levels in a variety of human biological samples. EMMPRIN, also known as CD147, holds a critical role in diverse cellular activities, particularly in modulating extracellular matrix remodeling and facilitating the expression of matrix metalloproteinases. By accurately measuring EMMPRIN/CD147 levels, researchers can gain valuable insights into its involvement in various physiological and pathological processes, including cancer progression, immune responses, and tissue homeostasis. Our ELISA kit provides exceptional sensitivity and specificity, ensuring the generation of consistent and reliable results crucial for unraveling the multifaceted functions of EMMPRIN/CD147. Crafted under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for cutting-edge research applications. Choose Assay Genie's EMMPRIN/CD147 ELISA Kit for accurate and dependable quantification of this pivotal biomarker in your research endeavors.
Product Name:
Human EMMPRIN/CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit
SKU:
AEES00159
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
100-107
92-100
101-108
Average (%)
103
95
105
1:4
Range (%)
85-96
89-95
85-98
Average (%)
91
92
92
1:8
Range (%)
101-109
95-104
88-101
Average (%)
103
98
94
1:16
Range (%)
91-102
89-100
101-109
Average (%)
96
93
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
94-105
98
Cell Culture Media (n=8)
100-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
0.86
3.44
0.55
0.84
3.68
Standard deviation
0.04
0.04
0.19
0.04
0.06
0.22
C V (%)
6.89
4.82
5.55
7.52
7.48
6.05
Uniport ID:
P35613
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human EMMPRIN/CD147 in samples.No significant cross-reactivity or interference between Human EMMPRIN/CD147 and analogues was observed
