The Human ENG (Endoglin) ELISA Kit offered by Assay Genie is meticulously designed for the precise quantitative detection of Endoglin (ENG) levels in diverse biological samples. Endoglin, a crucial component of vascular and tissue homeostasis, is involved in modulating cellular proliferation, differentiation, and migration. Notably, Endoglin plays a pivotal role in angiogenesis and endothelial function, making it a compelling target for cardiovascular and vascular studies. Our ENG ELISA Kit promises outstanding sensitivity and specificity, ensuring accurate and reproducible measurement of Endoglin concentrations. Crafted under stringent quality control standards, this kit delivers robust and dependable performance, offering researchers a valuable tool to advance their investigations. Trust in Assay Genie's ENG ELISA Kit for reliable and insightful results in your research endeavors.
Product Name:
Human ENG (Endoglin) ELISA Kit
SKU:
AEES00173
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
