Description
Human ENPP1 Recombinant Protein (N-His)
Human ENPP1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp362-Trp518, with an N-terminal His tag. Predicted molecular weight: 20.6 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1) is a ~104 kDa protein. Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. For research use only.
PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect.
| Product Name: | Human ENPP1 Recombinant Protein (N-His) |
| SKU: | GXRP03403 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, NPPS, PC1, NPPase, PDNP1, Membrane component chromosome 6 surface marker 1, ENPP1, Phosphodiesterase I/nucleotide pyrophosphatase 1, M6S1, Plasma-cell membrane glycoprotein PC-1, E-NPP 1, Nucleotide diphosphatase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp362-Trp518 |
| Calculated MW: | 20.6 kDa |
| UniProt: | P22413 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
