The Human ER Alpha (Estrogen Receptor Alpha) CLIA Kit is a highly sensitive assay designed for the precise measurement of ER Alpha levels in human biological samples. This kit offers exceptional specificity, ensuring accurate and reproducible results for a variety of research applications.Estrogen receptor alpha is a key regulator of estrogen signaling, playing a vital role in various physiological processes including cell growth, differentiation, and gene expression. Dysregulation of ER alpha has been implicated in numerous diseases such as breast cancer, osteoporosis, and cardiovascular disorders, making it a valuable biomarker for understanding disease pathogenesis and developing targeted therapies.
The Human ER Alpha CLIA Kit from Assay Genie provides researchers with a reliable tool to study the role of ER alpha in health and disease, offering unparalleled sensitivity and precision for the detection of this important protein. With its user-friendly format and quick assay time, this kit is an essential resource for laboratories conducting cutting-edge research in the field of estrogen receptor biology.
Product Name:
Human ER alpha (Estrogen Receptor Alpha) CLIA Kit
SKU:
HUES00662
Target:
Human ER alpha (Estrogen Receptor Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ERα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ERα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ERα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ERα. You can calculate the concentration of Human ERα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
96-112
89-101
Average (%)
93
103
95
1:4
Range (%)
101-119
96-111
102-116
Average (%)
110
103
108
1:8
Range (%)
87-102
88-100
92-109
Average (%)
93
95
99
1:16
Range (%)
96-109
84-99
91-104
Average (%)
103
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
100-112
106
Cell culture media (n=5)
84-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.58
84.75
444.05
48.27
81.15
406.26
Standard deviation
5.16
7.44
38.01
5.24
9.22
30.39
C V (%)
11.32
8.78
8.56
10.86
11.36
7.48
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ERα concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human ERα in samples. No significant cross-reactivity or interference between Human ERα and analogues was observed.
