Human ER Lipid Raft Associated Protein 2 (ERLIN2) ELISA Kit
The Human ERLIN2 (Erlin-2) ELISA Kit is specifically designed for the quantitative detection of ERLIN2 levels in human serum, plasma, and cell lysates. This kit provides researchers with a highly sensitive and specific tool to accurately measure ERLIN2 levels, ensuring reliable and reproducible results for a variety of research applications.ERLIN2 is a lipid raft-associated protein that plays a crucial role in various cellular processes, including protein degradation and lipid metabolism.
Dysregulation of ERLIN2 has been linked to various diseases, including cancer and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human ERLIN2 ELISA Kit is a valuable tool for researchers looking to study the role of ERLIN2 in disease pathogenesis and explore its potential as a therapeutic target.
Product Name:
Human ER Lipid Raft Associated Protein 2 (ERLIN2) ELISA Kit
Product Code:
HUDL00976
Size:
96 Assays
Target:
ERLIN2
Synonyms:
Erlin-2, SPFH2, NET32, C8orf2, ER Lipid Raft Associated Protein 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
