Description
Human ERCC3 Recombinant Protein (N-His)
Human ERCC3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Arg542-Ala702, with an N-terminal His tag. Predicted molecular weight: 20.91 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. General transcription and DNA repair factor IIH helicase/translocase subunit XPB (ERCC3) is a ~89 kDa protein. ATP-dependent 3'-5' DNA helicase/translocase. For research use only.
Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex. When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening; it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor.
| Product Name: | Human ERCC3 Recombinant Protein (N-His) |
| SKU: | GXRP03398 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | TFIIH basal transcription factor complex 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein, TFIIH subunit XPB, Basic transcription factor 2 89 kDa subunit, ERCC3, DNA excision repair protein ERCC-3, TFIIH p89, TFIIH 89 kDa subunit, DNA repair protein complementing XP-B cells, General transcription and DNA repair factor IIH helicase subunit XPB, XPB, BTF2 p89, XPBC |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Arg542-Ala702 |
| Calculated MW: | 20.91 kDa |
| UniProt: | P19447 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
