Description
Human ERCC5 Recombinant Protein (N-His)
Human ERCC5 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu778-Ala986, with an N-terminal His tag. Predicted molecular weight: 26.37 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA excision repair protein ERCC-5 (ERCC5) is a ~133 kDa protein. Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. For research use only.
Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes.
| Product Name: | Human ERCC5 Recombinant Protein (N-His) |
| SKU: | GXRP01353 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ERCC5, DNA excision repair protein ERCC-5, ERCM2, XPGC, Xeroderma pigmentosum group G-complementing protein, XPG, DNA repair protein complementing XP-G cells |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu778-Ala986 |
| Calculated MW: | 26.37 kDa |
| UniProt: | P28715 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
