Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit
The Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit is a valuable tool for the accurate quantification of EAAT3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.EAAT3 is a critical protein involved in the regulation of excitatory neurotransmission by facilitating the uptake of glutamate, an important neurotransmitter in the central nervous system. Dysregulation of EAAT3 has been implicated in various neurological disorders such as epilepsy, Alzheimer's disease, and Parkinson's disease, highlighting its potential as a biomarker for studying these conditions and exploring therapeutic interventions.
By utilizing the Human EAAT3 ELISA Kit, researchers can accurately measure EAAT3 levels in human samples, providing valuable insights into its role in neurological function and disease pathology. With its reliable performance and ease of use, this kit is an indispensable tool for advancing research in the field of neuroscience and drug discovery.
Product Name:
Human Excitatory Amino Acid Transporter 3 (EAAT3) ELISA Kit
Product Code:
HUDL00901
Size:
96 Assays
Target:
EAAT3
Synonyms:
SLC1A1, EAAC1, Solute Carrier 1 Member 1, Sodium-dependent glutamate/aspartate transporter 3, Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
