Description
Human F2/Prothrombin Recombinant Protein (N-His)
Human F2/Prothrombin Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr328-Glu622, with an N-terminal His tag. Predicted molecular weight: 36.10 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Prothrombin (F2/PIVKA-II) is a ~70 kDa protein. Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. For research use only.
Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces. Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells.
| Product Name: | Human F2/Prothrombin Recombinant Protein (N-His) |
| SKU: | GXRP06701 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | F2, Coagulation factor II, Prothrombin |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr328-Glu622 |
| Calculated MW: | 36.10 kDa |
| UniProt: | P00734 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
