The Human F9 (Coagulation Factor IX) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of Factor IX levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a variety of research applications in the fields of hemostasis and coagulation.Factor IX is a crucial protein involved in the coagulation pathway, playing a key role in blood clot formation. Deficiencies in Factor IX can lead to bleeding disorders such as hemophilia B, making it an important biomarker for studying these conditions and monitoring treatment outcomes.With its ease of use and accurate results, the Human F9 (Coagulation Factor IX) ELISA Kit is a valuable tool for researchers and clinicians seeking to understand and manage coagulation disorders.
Product Name:
Human F9 (Coagulation Factor IX) ELISA Kit
SKU:
HUES01902
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
