Human Family With Sequence Similarity 5, Member C (FAM5C) ELISA Kit
The Human Family with Sequence Similarity 5, Member C (FAM5C) ELISA Kit is a cutting-edge tool for the precise measurement of FAM5C levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results, making it a valuable asset for a diverse range of research endeavors.FAM5C, a member of the FAM5 gene family, plays a critical role in various biological processes, including cell migration, apoptosis, and tissue development.
Dysregulation of FAM5C has been implicated in numerous diseases, highlighting its importance as a potential biomarker for diagnostic and therapeutic purposes.The FAM5C ELISA Kit provides researchers with a powerful tool to investigate the role of FAM5C in health and disease, opening up new avenues for understanding its impact on cellular function and its potential as a target for future interventions.
Product Name:
Human Family With Sequence Similarity 5, Member C (FAM5C) ELISA Kit
Product Code:
HUDL01018
Size:
96 Assays
Target:
FAM5C
Synonyms:
DBCCR1L, DBCCR1L1, DBCCR1-like protein 1, BMP/retinoic acid-inducible neural-specific protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
