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Human Fas Ligand ELISpot Kit

SKU:
HUDC0145
Product type:
ELISpot
Reactivity:
Human
€306
Frequently bought together:

Description

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Human Fas Ligand ELISpot Kit

Assay Genie ELISpot is a highly specific immunoassay for the analysis of Fas Ligand production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of Fas Ligand producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted Fas Ligand (qualitative analysis) and single cells that produce Fas Ligand (quantitative analysis). Cell secreted Fas Ligand is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured Fas Ligand is revealed by tracer antibodies and appropriate conjugates.