Description
Human FEN1 Recombinant Protein (N-His)
Human FEN1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Thr336, with an N-terminal His tag. Predicted molecular weight: 40.02 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Flap endonuclease 1 (FEN1) is a ~42 kDa protein. Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. For research use only.
During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions.
| Product Name: | Human FEN1 Recombinant Protein (N-His) |
| SKU: | GXRP04529 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | FEN-1, Maturation factor 1, hFEN-1, DNase IV, RAD2, FEN1, Flap endonuclease 1, MF1, Flap structure-specific endonuclease 1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Thr336 |
| Calculated MW: | 40.02 kDa |
| UniProt: | P39748 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
