The Human Ferritin Light Polypeptide (FTL) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of FTL levels in human serum, plasma, and cell culture samples. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Ferritin is an important protein involved in iron storage and regulation, and abnormal levels of ferritin have been associated with various health conditions such as iron overload disorders, inflammatory diseases, and certain types of cancers.
Monitoring ferritin levels can provide valuable insights into the body's iron status and help in the diagnosis and management of related diseases.With its easy-to-use format and precise detection capabilities, the Human FTL ELISA Kit is a valuable tool for researchers studying iron metabolism, iron-related disorders, and the role of ferritin in various diseases. Trust in this reliable assay to support your research and advance our understanding of ferritin biology.
Product Name:
Human Ferritin, Light Polypeptide (FTL) ELISA Kit
Product Code:
HUDL01117
Size:
96 Assays
Target:
FTL
Synonyms:
Ferritin Light Chain, Ferritin Light Polypeptide-Like 3, L Apoferritin, Ferritin L Subunit
Detection Method:
Sandwich
Range:
31.2-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
