The Human Fibrinogen (FG) ELISA Kit is specifically designed for the quantitative determination of fibrinogen levels in diverse human biological samples. Fibrinogen is a key protein involved in blood coagulation, playing a crucial role in hemostasis, clot formation, and wound healing processes. Accurate measurement of fibrinogen levels is essential for assessing coagulation disorders, monitoring cardiovascular diseases, and evaluating inflammatory responses. Our FG ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for your research needs. Manufactured under stringent quality control guidelines, this kit guarantees robust performance and ease of use, making it a reliable choice for laboratory investigations. Trust Assay Genie's FG ELISA Kit for accurate and consistent quantification of this vital biomarker in your studies.
Product Name:
Human FG (Fibrinogen) ELISA Kit
SKU:
AEES00140
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
