Human FGF 23/Fibroblast Growth Factor 23 ELISA Kit
The Human FGF-23 (Fibroblast Growth Factor 23) ELISA Kit is specially designed to accurately measure FGF-23 levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring precise and consistent results that are vital for a variety of research purposes. FGF-23 is a critical protein that regulates phosphate and vitamin D metabolism, impacting bone health and cardiovascular function. Imbalances in FGF-23 levels have been linked to conditions like chronic kidney disease and mineral disorders, making it a valuable biomarker for studying these diseases and exploring potential treatments.
Overall, the Human FGF-23 ELISA Kit is an indispensable tool for researchers seeking to investigate the role of FGF-23 in health and disease, providing reliable data for further understanding its physiological functions and pathological implications.
Product Name:
Human FGF 23/Fibroblast Growth Factor 23 ELISA Kit
SKU:
HUFI02458
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
86-102%
86-98%
85-101%
EDTA Plasma (n = 5)
82-96%
83-97%
89-105%
Heparin Plasma (n = 5)
86-99%
82-90%
87-96%
Recovery:
Sample
1
2
Serum (n = 5)
86-99
95
EDTA Plasma (n = 5)
87-101
95
Heparin Plasma (n = 5)
90-99
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
