The Human FGF17 (Fibroblast Growth Factor 17) ELISA Kit is a powerful tool for accurately measuring FGF17 levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing researchers with reliable and consistent results for a variety of research applications. FGF17 is a key protein involved in cell growth, development, and tissue repair. It plays a crucial role in various biological processes including embryonic development, wound healing, and neural development.
Dysregulation of FGF17 has been linked to several diseases such as cancer, skeletal disorders, and neurological conditions, making it a valuable biomarker for studying and potentially treating these conditions. The Human FGF17 ELISA Kit from AssayGenie offers researchers a reliable and accurate method for quantifying FGF17 levels in human samples, enabling further insights into the role of this important protein in health and disease.
Product Name:
Human FGF17 ELISA Kit
SKU:
HUFI04293
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.938 pg/mL
Range:
1.563-100 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Cerebrospinal Fluid (CSF) , Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-99%
85-101%
85-99%
EDTA Plasma (n = 5)
89-103%
89-101%
80-95%
Heparin Plasma (n = 5)
90-103%
84-99%
88-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
92-104
98
EDTA Plasma (n = 5)
85-100
92
Heparin Plasma (n = 5)
91-101
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
