The Human Fibulin-4 (FBLN4) ELISA Kit is specifically designed for the accurate detection of fibulin-4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Fibulin-4 is a key extracellular matrix protein that plays a crucial role in maintaining tissue structure and function. Dysregulation of fibulin-4 has been linked to various diseases, including cardiovascular disorders, connective tissue diseases, and cancer.
Therefore, accurate measurement of fibulin-4 levels is essential for understanding its role in these conditions and developing potential therapeutic strategies.The Human Fibulin-4 ELISA Kit from Assay Genie provides researchers with a powerful tool for studying the role of fibulin-4 in health and disease. With its high sensitivity and easy-to-use format, this kit is a valuable asset for any laboratory conducting research in the field of extracellular matrix biology.
Product Name:
Human Fibulin 4 (FBLN4) ELISA Kit
Product Code:
HUDL01030
Size:
96 Assays
Target:
FBLN4
Synonyms:
EFEMP2, MBP1, UPH1, EGF-Containing Fibulin-Like Extracellular Matrix Protein 2
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
