Description
Human FOXK1 Recombinant Protein (N-His)
Human FOXK1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala86-Arg395, with an N-terminal His tag. Predicted molecular weight: 36.31 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Forkhead box protein K1 is a ~75 kDa protein. Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy. For research use only.
Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context. Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen. Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4. Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate.
| Product Name: | Human FOXK1 Recombinant Protein (N-His) |
| SKU: | GXRP09396 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Forkhead box protein K1; Myocyte nuclear factor; MNF; FOXK1; MNF |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala86-Arg395 |
| Calculated MW: | 36.31 kDa |
| UniProt: | P85037 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
