Human G Protein Coupled Receptor 78 (GPR78) ELISA Kit
The Human G Protein-Coupled Receptor 78 (GPR78) ELISA Kit is a cutting-edge tool designed for the precise detection of GPR78 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it invaluable for a variety of research applications.GPR78, also known as the lysophosphatidic acid receptor 5 (LPA5), plays a vital role in cellular signaling and has been implicated in numerous physiological processes, including cell migration, proliferation, and survival.
Dysregulation of GPR78 has been linked to various diseases, such as cancer, inflammatory disorders, and neurological conditions, highlighting its significance as a potential biomarker for disease diagnosis and therapy development.The Human GPR78 ELISA Kit offers researchers a powerful tool to investigate the role of GPR78 in health and disease, providing valuable insights to advance our understanding of molecular mechanisms and identify potential therapeutic targets. Trust in the accuracy and precision of this kit to elevate your research to new heights.
Product Name:
Human G Protein Coupled Receptor 78 (GPR78) ELISA Kit
Product Code:
HUDL01243
Size:
96 Assays
Target:
GPR78
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
