Human G6PD (Glucose 6 Phosphate Dehydrogenase) CLIA Kit
The Human G6PD (Glucose-6-Phosphate Dehydrogenase) CLIA Kit is specifically designed for the accurate quantification of G6PD levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for various research applications.G6PD is a key enzyme involved in the pentose phosphate pathway, playing a crucial role in maintaining cellular redox balance and protecting cells from oxidative damage.
Deficiencies in G6PD are linked to conditions such as hemolytic anemia and favism, making it an important biomarker for studying these disorders and developing potential treatments.With the Human G6PD CLIA Kit, researchers can accurately measure G6PD levels in human samples, gaining valuable insights into the pathophysiology of related diseases and exploring new therapeutic strategies.
Product Name:
Human G6PD (Glucose 6 Phosphate Dehydrogenase) CLIA Kit
SKU:
HUES01005
Target:
Human G6PD (Glucose 6 Phosphate Dehydrogenase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human G6PD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human G6PD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human G6PD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human G6PD. You can calculate the concentration of Human G6PD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
95-112
86-99
Average (%)
102
103
91
1:4
Range (%)
89-102
101-114
99-116
Average (%)
96
107
107
1:8
Range (%)
88-101
88-104
102-115
Average (%)
94
95
109
1:16
Range (%)
96-108
96-110
99-116
Average (%)
102
104
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
96-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
206.49
361.06
1587.89
209.52
373.1
1659.47
Standard deviation
20.19
38.53
140.69
26.97
32.68
124.13
C V (%)
9.78
10.67
8.86
12.87
8.76
7.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human G6PD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human G6PD in samples. No significant cross-reactivity or interference between Human G6PD and analogues was observed.
