The Human Gal3 (Galectin-3) ELISA Kit is specifically designed for the precise measurement of Gal3 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Gal3, also known as galectin-3, is a key player in various biological processes, including inflammation, fibrosis, and cancer development. Its overexpression has been linked to a number of diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human Gal3 ELISA Kit offers a reliable and efficient method for measuring Gal3 levels, making it an indispensable tool for researchers in the field of biomarker discovery and disease diagnostics.
Product Name:
Human GAL3 (Galectin 3) ELISA Kit
SKU:
HUES02501
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
