Human Galectin-1 PharmaGenie ELISA Kit (SBRS0604)

Product type:


Human Galectin-1 PharmaGenie ELISA - Kit Data

Product SKU:SBRS0604
Application:Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS-13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1, BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1, CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine, GITR Ligand, Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL-32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Siglec-7, Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2.
Gene ID:3956
Gene Names:LGALS1
Synonyms:Galectin-1 (Gal-1) (14 kDa laminin-binding protein) (HLBP14) (14 kDa lectin) (Beta-galactoside-binding lectin L-14-I) (Galaptin) (HBL) (HPL) (Lactose-binding lectin 1) (Lectin galactoside-binding soluble 1) (Putative MAPK-activating protein PM12) (S-Lac lectin 1)
Target Species:Human
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Range:4.10 ng/ml - 1,000 ng/ml
Sensitivity:4.10 ng/ml
Recommended Dilution:2 fold
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Stop Solution
4.Assay Diluent(s)
5.Lyophilized Standard
6.Biotinylated Detection Antibody
7.Streptavidin-Conjugated HRP
8.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human Galectin-1 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Kit Protocol:

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 ul of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 ul of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.