The Human Galectin-12 (Gal12) ELISA Kit is a reliable and accurate tool for detecting Galectin-12 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides trustworthy and consistent results, making it suitable for a variety of research applications.Galectin-12 is a protein that has been linked to inflammation and plays a role in various diseases such as obesity, diabetes, and atherosclerosis.
By studying Galectin-12 levels, researchers can gain insight into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Human Galectin-12 (Gal12) ELISA Kit is a valuable asset for researchers looking to investigate the role of Galectin-12 in human health and disease.
Product Name:
Human Galectin 12 (GAL12) ELISA Kit
Product Code:
HUDL01139
Size:
96 Assays
Target:
GAL12
Synonyms:
LGALS12, GRIP1, Lectin,Galactoside-Binding Soluble 12, Galectin-related inhibitor of proliferation
Detection Method:
Sandwich
Range:
0.312-20 ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
