The Human GC (Glucagon) ELISA Kit is specifically designed for the precise measurement of glucagon levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it an excellent choice for various research applications.Glucagon is a hormone that plays a critical role in regulating blood sugar levels by promoting the release of glucose from the liver. Imbalances in glucagon levels can lead to conditions such as diabetes and hypoglycemia, making it a valuable biomarker for studying metabolic disorders and developing new treatment strategies.Overall, the Human GC (Glucagon) ELISA Kit is a powerful tool for researchers looking to study the role of glucagon in health and disease, offering reliable and reproducible results for a wide range of experimental settings.
Product Name:
Human GC (Glucagon) ELISA Kit
SKU:
HUES03123
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
