Recombinant Human GITRL Protein(His Tag) (RPES5962)
Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1.Promotes leukocyte adhesion to endothelial cells .Regulates migration of monocytes from the splenic reservoir to sites of inflammation.
< 1.0 EU/mg of the protein as determined by the LAL method
Purity:
> 90% as determined by reducing SDS-PAGE.
Calculated MW:
14.6 kDa
Observed MW:
15 kDa
Bio-Activity:
Not validated for activity
Form:
Lyophilized powder
Source:
HEK293 Cells-derived Human GITRL protein Leu49-Ser177, with an C-terminal His
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Storage:
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to - 80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
