The Human GL Alpha Galactosidase Alpha ELISA Kit is designed for the precise measurement of GL Alpha Galactosidase Alpha levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.GL Alpha Galactosidase Alpha is an important enzyme involved in the breakdown of complex carbohydrates, specifically alpha-galactosidase. Deficiencies in this enzyme can lead to conditions such as Fabry disease, making it a valuable biomarker for studying these disorders and potential therapeutic interventions.With easy-to-follow instructions and quick assay times, the Human GL Alpha Galactosidase Alpha ELISA Kit is an essential tool for researchers and clinicians looking to accurately measure GL Alpha Galactosidase Alpha levels in biological samples.
Product Name:
Human GL alpha (Galactosidase Alpha) ELISA Kit
SKU:
HUES02093
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
