Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D) ELISA Kit
The Human Glutamate Receptor Ionotropic N-Methyl-D-Aspartate 2D (GRIN2D) ELISA Kit offered by Assay Genie is a cutting-edge tool for accurately measuring levels of GRIN2D in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results that are essential for a variety of research applications.GRIN2D is a critical component of the N-methyl-D-aspartate (NMDA) receptor, playing a pivotal role in excitatory neurotransmission in the central nervous system.
Dysregulation of GRIN2D has been implicated in various neurological disorders, including Alzheimer's disease, Parkinson's disease, and schizophrenia, highlighting its significance as a potential therapeutic target.By utilizing the Human GRIN2D ELISA Kit, researchers can gain valuable insights into the role of GRIN2D in neurological function and disease pathology, paving the way for the development of novel treatment strategies. Trust Assay Genie for accurate and reliable GRIN2D analysis, driving forward your research in neurobiology and beyond.
Product Name:
Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
