Description
Human GPBAR1 Recombinant Protein (N-His-KSI)
Human GPBAR1 Recombinant Protein (N-His-KSI) is a recombinant protein expressed in E. coli, spanning [Thr2-Gly19] & [Pro72-Cys85] & [Asn147-Tyr165] & [Ala250-Thr261] & [Gly283-Asn330], with an N-terminal His-KSI tag. Predicted molecular weight: 28.29 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. G-protein coupled bile acid receptor 1 (GPBAR1) is a ~35 kDa protein. G protein-coupled receptor for bile acid. For research use only.
Bile acid-binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPBAR1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. Activated by bile acids, such as lithocholate, deoxycholate, chenodeoxycholate and cholate, in descending order. Apart from their role in lipid dietary absorption and cholesterol catabolism, bile acids act as an important signaling molecule, involved in processes, such as energy expenditure or tissue inflammation.
| Product Name: | Human GPBAR1 Recombinant Protein (N-His-KSI) |
| SKU: | GXRP07708 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, hGPCR19, Membrane-type receptor for bile acids, M-BAR, hBG37, BG37, GPBAR1, TGR5 |
| Tag: | N-His-KSI |
| Expression Host: | E. coli |
| Protein Length: | [Thr2-Gly19] & [Pro72-Cys85] & [Asn147-Tyr165] & [Ala250-Thr261] & [Gly283-Asn330] |
| Calculated MW: | 28.29 kDa |
| UniProt: | Q8TDU6 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
