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Human Granzyme B ELISpot Kit

SKU:
HUDC0146
Product Type:
ELISpot
Reactivity:
Human
€355
Frequently bought together:

Description

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Human Granzyme B ELISpot Kit

Assay Genie ELISpot is a highly specific immunoassay for the analysis of Granzyme B production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of Granzyme B producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted Granzyme B (qualitative analysis) and single cells that produce Granzyme B (quantitative analysis). Cell secreted Granzyme B is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured Granzyme B is revealed by tracer antibodies and appropriate conjugates.