Human GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit
The Human Gro-alpha (CXCL1) ELISA Kit is specifically designed for the precise measurement of growth-regulated oncogene alpha levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes. Gro-alpha, also known as CXCL1, is a critical chemokine involved in inflammation and immune response regulation. Its role in promoting cell proliferation and migration makes it a crucial player in diseases such as cancer, inflammatory disorders, and autoimmune conditions. As a valuable biomarker, the Gro-alpha ELISA Kit provides researchers with a powerful tool for studying these conditions and exploring potential therapeutic interventions.
Product Name:
Human GRO alpha/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit
SKU:
HUES01332
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.