Human Growth Differentiation Factor 10 (GDF10) ELISA Kit
The Human Growth Differentiation Factor 10 (GDF10) ELISA Kit is a cutting-edge tool designed for the precise measurement of GDF10 levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results across various research applications.GDF10, also known as BMP-3b, is a critical factor involved in regulating cell growth, differentiation, and development. This protein plays a vital role in various physiological processes, including bone and cartilage formation, making it a key target for studying conditions such as osteoporosis and skeletal disorders.
By utilizing the Human GDF10 ELISA Kit, researchers can unlock valuable insights into the role of GDF10 in disease progression and possibly identify new therapeutic targets for treating bone-related disorders. Trust in the precision and reliability of this advanced ELISA kit to propel your research forward and expand our understanding of GDF10 biology.
Product Name:
Human Growth Differentiation Factor 10 (GDF10) ELISA Kit
Product Code:
HUDL01164
Size:
96 Assays
Target:
GDF10
Synonyms:
BMP3b, BIP, Bone-inducing protein, Bone morphogenetic protein 3B
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
